RNA Sequencing of Type 2 Alveolar Epithelial Cells in Coculture with Human Umbilical Cord-Derived Mesenchymal Stem Cells (hUC-MSCs) after Lipopolysaccharide Exposure
This dataset provides the RNA sequencing of MLE-12 cells exposed to lipopolysaccharides (LPS) at 0 (control group) or 1 (LPS group) μg/mL for 2 hours, followed by coculturing with human umbilical cord-derived mesenchymal stem cells (hUC-MSCs; LPS + MSC group) for cell collection at 6 or 12 hours. The dataset includes the summary of gene quantification, differentially expressed genes (DEG), and gene annotation from Disease Ontology (DO), Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG).
Steps to reproduce
The MLE-12 cells (ATCC, Manassas, VA, USA) were exposed to 0 or 1 µg/mL LPS (Sigma-Aldrich, St. Louis, MO, USA) for 2 hours and cocultured with hUC-MSCs for cell lysate collection at 6 and 12 hours. Cell lysates were conserved by adding 1 mL of TRIzol™ reagent (Sigma-Aldrich) in each sample and stored at -80°C. The purification and quantification of RNA were analyzed by SimpliNano™–Biochrom Spectrophotometer (Biochrom, Holliston, MA, USA). Qsep 100 DNA/RNA Analyzer (BiOptic, New Taipei City, Taiwan) was conducted to determine RNA integrity and degradation, and NovaSeq 6000 (Illumina, San Diego, CA, USA) was used for sequencing. Gene expression without biological duplicate was analyzed from DEG-sequencing (DEGseq), DO, GO, and KEGG databases via the RNASeq platform (BIOTOOLS, New Taipei City, Taiwan).